FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442658989
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG5055690.11421184536252577TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1663850.057.4272771
TCGGAAG1811950.052.613292
CGGAAGA2043350.046.733373
AGAGCAC4533250.021.6194327
AGCACAC4841400.020.1954699
GAGCACA4947300.019.802738
GAAGAGC5109450.019.242385
TATGCCG1071400.018.23782545-49
CGTATGC1192900.016.2587245-49
CTCGTAT1091000.015.54202640-44
TCTCGTA1135150.014.92732440-44
CGCTCAT1323050.014.69611230-34
ATGCCGT1369000.014.41192545-49
TGCCGTC1374150.014.0572345-49
TATCTCG1198650.013.98050540-44
ACCGCTC1388300.013.91872230-34
GCCGTCT1411200.013.65154250-54
GTCACCG1450300.013.573565525-29
AAGAGCA7526450.013.4174026
TCGTATG1262650.013.29943240-44
Produced by FastQC (version 0.11.5)