FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442658989
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC6164380.1392579875973105Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1649150.058.4272049
GAGCGTC2023200.047.7501148
ATCGGAA2222900.044.2447931
TCGGAAG2371350.041.408952
AGAGCGT2388150.040.6379057
CGGAAGA2616600.037.4690553
AAGAGCG2834450.034.5590936
GAAGAGC5642400.017.8832045
CGCCGTA1113950.017.67376955-59
TCGCCGT1261800.015.65571355-59
GCCGTAT1362050.014.66306555-59
GTCGCCG1399100.014.075829555-59
CCGTATC1485350.013.26824755-59
TGGTCGC1540500.012.99851150-54
CGTATCA1576500.012.64850860-64
GCGTCGT1556350.012.63822410-14
GGTCGCC1571500.012.5446950-54
CGTCGTG1615750.012.23640510-14
GTGGTCG1679800.012.02328750-54
GGAAGAG9312750.011.2280974
Produced by FastQC (version 0.11.5)