FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465062530
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG7455210.16030553998835384TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2016800.066.435471
TCGGAAG2176050.061.4268652
CGGAAGA2387750.056.0119863
AGAGCAC5067650.026.9103037
AGCACAC5359350.025.4753889
GAGCACA5517250.024.7712488
GAAGAGC5620400.024.322975
TATGCCG1371950.020.06111545-49
CGTATGC1509450.018.07131245-49
CTCGTAT1379450.017.9970640-44
TCTCGTA1431100.017.28159140-44
AAGAGCA8175000.017.0299536
ATGCCGT1662500.016.59435345-49
TGCCGTC1693000.015.81823945-49
GCCGTCT1731050.015.64311550-54
TCGTATG1573250.015.40638440-44
GGAAGAG9364650.015.010764
ATCTCGT1672450.014.90835240-44
ACACGTC2004700.013.86650110-14
CGTCTGA2001150.013.83701415-19
Produced by FastQC (version 0.11.5)