FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454112174
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC12493400.2751170463005469Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3409100.059.065939
GAGCGTC3797600.053.1003768
AGAGCGT4122050.048.955927
ATCGGAA4183100.048.6840021
TCGGAAG4259100.047.65922
CGGAAGA4546550.044.687063
AAGAGCG4639050.043.802946
GAAGAGC7548050.027.308415
CGCCGTA2193400.019.23388555-59
GGAAGAG10998850.019.061644
TCGCCGT2423500.017.47472455-59
GCCGTAT2445400.017.3680355-59
TGGTCGC2544350.016.6752750-54
GTCGCCG2587250.016.29979755-59
CGTATCA2598350.016.24751560-64
CCGTATC2537400.015.94233355-59
GTGGTCG2675200.015.927450-54
GGTCGCC2656050.015.27519950-54
CCGTGTA2950400.014.49296835-39
TCTCGGT3024250.014.21138245-49
Produced by FastQC (version 0.11.5)