FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488443550
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC31868480.6524496024156733Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4616000.0108.673999
GAGCGTC5011800.0100.225518
ATCGGAA5255150.099.144071
TCGGAAG5496650.094.709882
AGAGCGT5378750.093.53267
CGGAAGA5756350.090.31393
AAGAGCG6065850.085.335366
GAAGAGC9336550.055.8436165
TCCGATC838700.046.909753
TTCCGAT870450.045.379762
GGAAGAG13655200.038.626124
CCGATCT1008000.037.650134
CTTCCGA1071050.036.9705351
AGCGGCG779550.025.9445119
CGCCGTA3918950.025.10166755-59
TCGCCGT3984650.024.1976355-59
GCCGTAT4174650.023.6878255-59
GTCGCCG4223150.023.31758955-59
CGATCTA443750.023.2965435
CCGTATC4295500.022.93969755-59
Produced by FastQC (version 0.11.5)