FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442236589
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG17135720.38747856749591564TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4095900.070.316951
TCGGAAG4222900.068.220392
CGGAAGA4425750.065.0323
AGAGCAC6797200.042.578567
AGCACAC7068600.040.794319
GAGCACA7182500.040.3168268
GAAGAGC7378200.039.3454635
AAGAGCA9835900.029.7542746
GGAAGAG10998200.026.750024
TATGCCG2536300.023.11556645-49
CTCGTAT2594450.021.67487540-44
CGTATGC2708250.021.38413445-49
TCTCGTA2654550.021.14757240-44
ATGCCGT2823400.020.82560345-49
TGCCGTC2805300.020.58338745-49
GCCGTCT2832000.020.45292750-54
TATCTCG2730650.020.32419440-44
TCGTATG2734200.020.2119540-44
CGCTCAT2917150.019.97797830-34
ATCTCGT2881500.019.56594840-44
Produced by FastQC (version 0.11.5)