FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432467941
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG11832420.27360224604486927TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3334950.057.5640451
TCGGAAG3436900.055.933172
CGGAAGA3610950.053.228663
AGAGCAC5949000.032.5386737
AGCACAC6233650.031.0400569
GAGCACA6327350.030.6262118
GAAGAGC6563350.029.7284185
AAGAGCA9056100.021.8025476
TATGCCG1858850.021.04535945-49
GGAAGAG10138050.019.5962754
CTCGTAT1896950.019.57719840-44
CGTATGC1995550.019.39738545-49
TCTCGTA1942200.019.11511240-44
TGCCGTC2072900.018.59874345-49
ATGCCGT2120950.018.4890745-49
GCCGTCT2101100.018.37213350-54
TCGTATG2042700.017.94046640-44
ATCTCGT2166850.017.2122840-44
CGTCTTC2353800.016.7095250-54
CCGTCTT2402050.016.36180550-54
Produced by FastQC (version 0.11.5)