FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432467941
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC12396930.28665546794831664Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3115200.061.6787769
GAGCGTC3463850.055.5245328
AGAGCGT3876600.049.7456137
ATCGGAA3943050.049.4498141
TCGGAAG4042400.048.185312
CGGAAGA4233900.045.948183
AAGAGCG4329500.044.7930536
GAAGAGC7133550.027.5826995
CGCCGTA1809950.021.38816655-59
TCGCCGT1918750.020.17160455-59
GCCGTAT2031000.019.11528855-59
GGAAGAG10524400.019.0509174
GTCGCCG2046500.018.860755-59
TGGTCGC2175450.018.0017650-54
CCGTATC2160250.017.77830955-59
GGTCGCC2219400.017.41858950-54
CGTATCA2234300.017.32360-64
GTGGTCG2302000.017.07826850-54
GTACGTC2399550.016.03924230-34
TACGTCC2482750.015.66349730-34
Produced by FastQC (version 0.11.5)