FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468230537
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1334950.048.6776281
TCGGAAG1509100.043.1312642
CGGAAGA1722100.037.8532873
TATGCCG858850.015.66272945-49
AGAGCAC4417900.015.2128727
AGCACAC4745850.014.2972629
GAGCACA4886150.013.9238268
GAAGAGC5006500.013.5720015
CGTATGC998650.013.51366545-49
CTCGTAT931100.013.21114640-44
TCTCGTA978900.012.62527440-44
ATGCCGT1183350.011.59429145-49
TGCCGTC1188950.011.24707545-49
GCCGTCT1225900.011.12847450-54
TCGTATG1095200.011.12708640-44
ATCTCGT1230250.010.22256840-44
CGTCTGA1394000.09.92520415-19
ACACGTC1406000.09.85908110-14
AAGAGCA7560850.09.2955186
CGTCTTC1527200.09.15411150-54
Produced by FastQC (version 0.11.5)