FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468230537
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1331500.048.9008679
GAGCGTC1715950.038.1855858
ATCGGAA1825000.036.431421
TCGGAAG2014250.033.0021172
AGAGCGT2034850.032.27877
CGGAAGA2235100.029.7986683
AAGAGCG2467550.026.9271266
CGCCGTA854000.015.71080955-59
TCGCCGT1009450.013.41925655-59
GAAGAGC5521200.012.5752745
GCCGTAT1108550.012.38180555-59
GTCGCCG1141350.011.94213855-59
CCGTATC1240000.010.87630955-59
TGGTCGC1271050.010.85615250-54
GCGTCGT1244950.010.76018310-14
CGTATCA1329600.010.43538960-64
CGTCGTG1307400.010.29832510-14
GGTCGCC1326100.010.22726550-54
GTGGTCG1399950.09.95910650-54
TCGTGTA1582200.08.70577710-14
Produced by FastQC (version 0.11.5)