FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474873909
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT156102003.2872305056456574TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG10724900.22584732066212548TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA17458300.0140.73931
ATCGGAA19226350.0128.211332
TCGGAAG19414750.0126.846543
CGGAAGA19664950.0125.1250844
AGAGCAC22373000.0109.6650858
GAGCACA22796100.0107.538129
GAAGAGC22922000.0107.2660456
AAGAGCA25453050.096.6215747
GGAAGAG26600150.092.648485
CGCGAAA18534950.028.35064735-39
TATGCCG18765550.028.23553345-49
TCCGCGA18607000.028.23295830-34
TCTCGTA18602250.028.01438940-44
GCGAAAT18827200.027.96870835-39
CTCGTAT18564550.027.96795840-44
CGTATGC18908150.027.94675645-49
CTCCGCG18999500.027.74492830-34
ATGCCGT19066350.027.67008445-49
ACTCCGC19062650.027.66624630-34
ATCTCGT18841350.027.665540-44
Produced by FastQC (version 0.11.5)