FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432048279
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG6968580.16129169675502864TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1939250.070.412881
TCGGAAG2086300.065.368092
CGGAAGA2270250.059.9878653
AGAGCAC4705850.029.3208317
AGCACAC5094950.027.0688069
GAGCACA5136900.026.8731528
GAAGAGC5224250.026.579595
TATGCCG1366800.019.9176645-49
AAGAGCA7686150.018.3836566
CGTATGC1481700.018.2067845-49
CTCGTAT1343350.017.66221640-44
TCTCGTA1384700.017.17666840-44
CGCTCAT1598750.016.99278630-34
ATGCCGT1617500.016.71494945-49
GGAAGAG8574800.016.531084
ACCGCTC1652150.016.48392130-34
TGCCGTC1619450.016.44056745-49
TATCTCG1445650.016.28399740-44
GCCGTCT1656800.015.92744450-54
TCGTATG1465500.015.86655940-44
Produced by FastQC (version 0.11.5)