FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463625367
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1774350.038.680739
GAGCGTC2151050.031.9698338
ATCGGAA2353900.029.7270571
AGAGCGT2474400.027.9577797
TCGGAAG2521200.027.6583862
CGGAAGA2725650.025.6310753
AAGAGCG2914150.023.8757386
CGCCGTA1049700.013.36573455-59
GAAGAGC5963600.012.1935485
TCGCCGT1202200.011.7197355-59
GCCGTAT1293500.011.020355-59
GTCGCCG1353850.010.46157955-59
CCGTATC1413700.09.93252655-59
TGGTCGC1452450.09.91605450-54
CGTATCA1491900.09.63831760-64
GGTCGCC1524850.09.291224550-54
GTGGTCG1602550.09.05874750-54
CAGTACG1621650.08.65108130-34
GCGTCGT1710950.08.21538710-14
AGTACGT1751350.08.20945735-39
Produced by FastQC (version 0.11.5)