FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450620785
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG8426180.18699048691240464TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1891000.074.482411
TCGGAAG2060150.068.355162
CGGAAGA2278700.061.7394683
AGAGCAC4883750.029.2724257
AGCACAC5210950.027.4482969
GAGCACA5283450.027.1169288
GAAGAGC5387600.026.5884515
TATGCCG1436200.020.51998945-49
TCGTATC1577800.018.72118835-39
CTCGTAT1478250.018.71605340-44
CGTATGC1561050.018.67821745-49
AAGAGCA7900900.018.4865746
TCTCGTA1511300.018.29812440-44
GTCACGA1686450.017.54159425-29
ATGCCGT1715300.017.23439245-49
TATCTCG1598000.017.15927340-44
GGAAGAG8916650.016.5092834
ATTCGTA1788350.016.44490635-39
GCCGTCT1774150.016.3453150-54
TGCCGTC1734600.016.33214245-49
Produced by FastQC (version 0.11.5)