FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500410510
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC33473350.6689178051036538Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4911800.0106.5018549
GAGCGTC5311100.098.586248
ATCGGAA5483700.096.768391
TCGGAAG5761050.092.207072
AGAGCGT5694950.092.064687
CGGAAGA6004750.088.443733
AAGAGCG6283850.083.986956
GAAGAGC9646450.055.2461475
GGAAGAG14064750.038.2917824
CGCCGTA4275750.024.64231555-59
TCGCCGT4406150.023.86992855-59
GCCGTAT4549700.023.2652755-59
GTCGCCG4558550.023.02612555-59
CCGTATC4647600.022.56684355-59
TGGTCGC4726150.022.32042550-54
CGTATCA4714600.022.20925560-64
GGTCGCC4757850.022.06263550-54
GCGTCGT4835150.021.67678310-14
GTGGTCG4919900.021.4994750-54
CGTCGTG4903150.021.46958410-14
Produced by FastQC (version 0.11.5)