FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446036365
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC5487630.12303099994997045Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1795600.048.4654669
GAGCGTC2215150.039.6425368
ATCGGAA2315150.038.7415471
TCGGAAG2486400.035.9173742
AGAGCGT2486300.035.4299437
CGGAAGA2717700.032.768643
AAGAGCG2946650.030.2422126
GAAGAGC5666200.016.2678975
CGCCGTA1154250.015.4249755-59
TCGCCGT1348700.013.26017255-59
GCCGTAT1397400.012.94845555-59
GTCGCCG1504950.011.93256855-59
CCGTATC1526650.011.70219155-59
CGTATCA1577750.011.45486460-64
TGGTCGC1640700.011.12498350-54
GGTCGCC1692000.010.67032350-54
GGAAGAG9199850.010.405494
GTGGTCG1796300.010.21617850-54
GCGTCGT1787550.09.9739310-14
CGTCGTG1845150.09.68615910-14
Produced by FastQC (version 0.11.5)