FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447510226
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG4671940.10439850820302819TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1495600.058.639691
TCGGAAG1634550.053.6920282
CGGAAGA1859450.047.26043
CGCGCAT834600.020.83420835-39
AGAGCAC4405350.020.523157
TCGCGCA852200.020.45668235-39
TCTCGCG905200.019.53584930-34
AGCACAC4736050.019.0426529
GAGCACA4853000.018.6136268
GAAGAGC4960400.018.2803545
TATGCCG990950.018.09091445-49
GCGCATC1023800.017.21913535-39
CTCGCGC1073300.016.1775930-34
CGTATGC1116900.015.94697445-49
CTCGTAT1011600.015.34766240-44
TCTCGTA1047350.014.74764240-44
ATGCCGT1251050.014.42244945-49
ACTCTCG1257100.014.34400230-34
TGCCGTC1293800.013.59058245-49
GCCGTCT1331550.013.31690150-54
Produced by FastQC (version 0.11.5)