FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470967069
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC14909340.31656863040672595Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2528600.090.727829
GAGCGTC2836700.080.95548
ATCGGAA3035650.076.365791
TCGGAAG3193650.072.524052
AGAGCGT3283600.070.195487
CGGAAGA3477950.066.745133
AAGAGCG3706950.062.3469166
TTCCGAT976450.057.8743862
TCCGATC981400.057.632753
CTTCCGA1126800.050.1949421
CCGATCT1127250.048.8832744
CGATCTA502900.035.7547655
GAAGAGC6776600.034.608675
CGCCGTA2058050.022.81070955-59
GGAAGAG10677300.022.4158444
TCGCCGT2147000.021.81437555-59
GTCGCCG2188450.021.15821655-59
GCCGTAT2323350.020.31512355-59
GGTCGCC2378950.019.75380950-54
TGGTCGC2410850.019.57478150-54
Produced by FastQC (version 0.11.5)