FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387693605
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG8287550.21376545532650715TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2943950.045.3864361
TCGGAAG2995050.044.6351782
CGGAAGA3142850.042.515063
AGAGCAC5172000.026.16357
AGCACAC5377250.025.2010869
GAGCACA5495600.024.6793928
GAAGAGC5692450.023.9083945
TATGCCG1422350.019.25295345-49
CGTATGC1551500.017.53303345-49
CTCGTAT1477700.017.41668140-44
AAGAGCA7983650.017.2974996
TCTCGTA1514500.017.01636540-44
GCCGTCT1644900.016.7727250-54
TGCCGTC1611900.016.71569645-49
ATGCCGT1654300.016.64862845-49
TATCTCG1592400.016.02705840-44
GGAAGAG8833750.015.7866794
TCGTATG1624700.015.58400640-44
ATCTCGT1715400.015.131227540-44
CGTCTTC1882400.014.95775950-54
Produced by FastQC (version 0.11.5)