FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387693605
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC8839980.22801459415354555Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2591050.051.7316259
GAGCGTC2904100.046.203148
AGAGCGT3242050.041.522447
ATCGGAA3403800.039.9009861
TCGGAAG3466500.039.0967452
CGGAAGA3614100.037.4937363
AAGAGCG3617150.037.3702746
GAAGAGC6116350.022.506795
CGCCGTA1384800.019.78525455-59
TCGCCGT1475300.018.5764755-59
GTCGCCG1566950.017.4224355-59
GCCGTAT1598050.017.2701855-59
TGGTCGC1690400.016.37455750-54
CCGTATC1686300.016.0655855-59
GGTCGCC1708850.015.86024150-54
CGTATCA1761400.015.6145660-64
GTGGTCG1789900.015.50478350-54
GGAAGAG9137250.015.4271594
CAGTACG1965450.013.7030230-34
AGTACGT2071350.013.27286735-39
Produced by FastQC (version 0.11.5)