FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392958254
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG12399680.31554700464441704TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3239800.064.1462251
TCGGAAG3307300.062.8332372
CGGAAGA3464500.059.9429933
AGAGCAC5581500.037.4627727
AGCACAC5808800.035.934459
GAGCACA5940400.035.1688888
GAAGAGC6124350.034.245125
AAGAGCA8436800.025.1062936
GGAAGAG9315150.022.9074364
TATGCCG1920850.022.02990745-49
GCGCATC2005100.020.94318835-39
CGTATGC2045750.020.4871845-49
CTCGTAT1926550.020.4212240-44
TAATGCG2122650.019.95584730-34
TCTCGTA1968800.019.93585440-44
ATGCCGT2143750.019.73728445-49
TGCCGTC2119450.019.5647745-49
TGCGCAT2155550.019.51976435-39
AATGCGC2140350.019.4074430-34
GCCGTCT2148750.019.40724450-54
Produced by FastQC (version 0.11.5)