FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409314315
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG11641030.2844031975769037TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3934250.049.567461
TCGGAAG4001300.048.7401472
CGGAAGA4154000.047.0096553
AGAGCAC6284400.031.2638827
AGCACAC6460600.030.3876729
GAGCACA6600000.029.784278
GAAGAGC6902800.028.6893845
AAGAGCA9240950.021.6436356
GGAAGAG10236500.019.6590044
TATGCCG2078250.019.07764445-49
CGCGAAA2117650.018.47815135-39
TCCGCGA2182300.018.11251430-34
CGTATGC2183600.017.90701345-49
CTCGTAT2120400.017.73093240-44
CCGCGAA2182400.017.72456430-34
TCTCGTA2180650.017.28224440-44
ATGCCGT2336650.017.04731645-49
TGCCGTC2317300.016.81442645-49
TCGTATG2224250.016.55124340-44
GCCGTCT2388650.016.4890650-54
Produced by FastQC (version 0.11.5)