FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414041970
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC41741131.008137653291525Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG7224950.091.5731669
GAGCGTC7610900.086.916128
AGAGCGT8007450.082.7827
ATCGGAA8289200.080.476481
TCGGAAG8337400.079.991822
AAGAGCG8498350.078.331146
CGGAAGA8526250.078.268983
GAAGAGC11136050.060.0978135
GGAAGAG14271950.047.1285974
CGCCGTA5170550.025.66500955-59
TCGCCGT5299100.024.96114355-59
GCCGTAT5374600.024.72865755-59
GTCGCCG5449700.024.24474755-59
CCGTATC5470450.024.08997355-59
TGGTCGC5589350.023.84110650-54
CGTATCA5529500.023.83406460-64
GGTCGCC5588700.023.67706750-54
GTGGTCG5736300.023.26523450-54
TCTCGGT6080150.022.04885945-49
TCGGTGG6122900.021.68677345-49
Produced by FastQC (version 0.11.5)