FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395444951
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG34838090.8809845697081615TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5196950.0108.76011
TCGGAAG5366700.0105.753852
CGGAAGA5546450.0102.3553853
AGAGCAC7701500.073.82047
AGCACAC7934200.071.538389
GAGCACA8041750.070.665478
GAAGAGC8213050.069.3990555
AAGAGCA10425400.054.827066
GGAAGAG11411950.050.21634
TCCGCGA4227150.026.90902930-34
CGCGAAA4192400.026.78425435-39
TATGCCG4298350.026.59871345-49
CCGCGAA4249050.026.53728330-34
CTCGTAT4272250.025.8766340-44
TCTCGTA4311100.025.63570240-44
CGTATGC4432100.025.46428145-49
CTCCGCG4479450.025.43936730-34
GCGAAAT4476550.025.38209235-39
ATGCCGT4544000.025.1425945-49
TGCCGTC4547300.024.88773345-49
Produced by FastQC (version 0.11.5)