FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28583145
Sequences flagged as poor quality0
Sequence length76-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4485861.5694074252500905TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG1652200.5780329631326434TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA939450.030.43062862-63
GCTTGAA956250.029.97206160-61
GAAAAAA1078700.026.58872864-65
GCCGTCT1081800.026.49712450-51
ATGCCGT1122150.025.56362548-49
CGTCTTC1129500.025.41305552-53
CTGCTTG1158150.024.91869558-59
GTATGCC1169300.024.5169946-47
TCGTATG1191300.024.01061844-45
TTCTGCT1251550.023.09370656-57
CTTGAAA931850.022.37487862-63
TGAAAAA951900.021.97871664-65
TCTCGTA1273550.021.49419642-43
TGCTTGA1001050.020.9547160-61
TCTTCTG1408100.020.60886854-55
AATCTCG1387900.019.70901940-41
TATGCCG1090550.019.12954548-49
CCGTCTT1146200.018.31762752-53
TGCCGTC1146500.018.28045850-51
CGTATGC1161750.017.93411846-47
Produced by FastQC (version 0.11.5)