FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24894027
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2082080.8363773366197442TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG460820.18511267783231697TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA504900.023.92065862-63
GCTTGAA514150.023.62374960-61
GCCGTCT552100.021.85295950-51
ATGCCGT574050.021.0511348-49
GAAAAAA589300.020.67755564-65
CTTGAAA501100.020.38875662-63
GTATGCC593300.020.31994446-47
CGTCTTC604700.020.02378752-53
TCGTATG602000.019.96956644-45
TGAAAAA514350.019.93899564-65
TGCTTGA544300.018.86898460-61
TATGCCG540300.018.746748-49
CTGCTTG674700.018.20063658-59
TGCCGTC593300.017.24628450-51
CGTATGC589250.017.1629746-47
TCTCGTA678950.016.85581842-43
CCGTCTT616350.016.57725352-53
TTCTGCT774200.015.97559456-57
CTCGTAT638250.015.700644544-45
GATCTCG772800.014.86999740-41
Produced by FastQC (version 0.11.5)