FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26710902
Sequences flagged as poor quality0
Sequence length76-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4341321.625298913529764TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG2146510.8036082046199713TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA957650.031.4479962-63
GCTTGAA991200.030.452760-61
GAAAAAA1095950.027.59568264-65
GCCGTCT1099250.027.4923850-51
ATGCCGT1140100.026.50553348-49
CGTCTTC1152650.026.24016652-53
GTATGCC1163600.025.9651946-47
CTGCTTG1198850.025.3529758-59
TCGTATG1191450.025.31938644-45
TTCTGCT1301850.023.45653256-57
TCTCGTA1286300.022.6554342-43
TATCTCG1320200.022.04906340-41
TCTTCTG1402100.021.74272754-55
CTTGAAA960000.021.08293362-63
CGTATCT1406550.020.71946338-39
TGAAAAA982850.020.59737464-65
TGCTTGA1048450.019.47041360-61
TTCGTAT1511850.019.33903336-37
TATGCCG1099850.018.40314148-49
AATTCGT1651700.018.27136834-35
Produced by FastQC (version 0.11.5)