FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27541627
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4079631.481259622025961TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG971120.35260081040237745TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA794700.029.50699462-63
GCTTGAA824000.028.6089660-61
GAAAAAA915500.025.80909264-65
GCCGTCT925600.025.36766450-51
ATGCCGT965500.024.32825548-49
CTTGAAA797150.024.10547862-63
TGAAAAA809500.023.76452864-65
GTATGCC1002650.023.40659146-47
CGTCTTC1008450.023.3139452-53
CTGCTTG1020950.023.17721758-59
TCGTATG1037650.022.59091644-45
TGCTTGA863250.022.35417260-61
TTCTGCT1149900.020.70938956-57
TATGCCG939150.020.38123948-49
TCTCGTA1150250.019.7048142-43
TGCCGTC979400.019.64043450-51
TATCTCG1178350.019.20732740-41
CGTATGC1009500.018.97297946-47
TCTTCTG1265900.018.86442254-55
CCGTCTT1020950.018.84993752-53
Produced by FastQC (version 0.11.5)