FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27392633
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2249040.8210382696690749TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG603170.22019423981623087TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA579700.023.20042460-61
TTGAAAA582250.022.88842462-63
GCCGTCT648100.020.52023550-51
ATGCCGT695750.019.11187248-49
CGTCTTC707300.018.91894352-53
CTTGAAA566400.018.85430562-63
GAAAAAA717400.018.73856764-65
GTATGCC732750.018.13434246-47
CTGCTTG752800.017.94914658-59
TGAAAAA605200.017.65268364-65
TGCTTGA614100.017.50522260-61
TCGTATG759550.017.4524644-45
TATGCCG662350.015.92529148-49
TTCTGCT898900.015.13490756-57
TGCCGTC711950.014.98202850-51
CCGTCTT714350.014.93515652-53
TCTCGTA882750.014.45810842-43
CGTATGC732250.014.38097946-47
ACGTTCG26800.014.2965866
CGTTCGA28400.013.9461317
Produced by FastQC (version 0.11.5)