FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25675783
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4775661.8599861199948604TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG1681090.6547375789863935TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA953750.031.50093562-63
GCTTGAA978800.030.76684660-61
GCCGTCT1062700.028.33730150-51
GAAAAAA1074550.028.05395164-65
ATGCCGT1105500.027.21112848-49
CGTCTTC1121350.026.87469552-53
GTATGCC1137050.026.46444546-47
CTGCTTG1150600.026.30272358-59
TCGTATG1166450.025.72964144-45
TTCTGCT1268650.023.98060456-57
CTTGAAA954450.023.53175462-63
TGAAAAA959950.023.40609664-65
TCTCGTA1250350.023.18891342-43
TATCTCG1285000.022.55161940-41
TGCTTGA1008950.022.36480360-61
TCTTCTG1372300.022.15360654-55
CGTATCT1371750.021.13611638-39
TATGCCG1078150.020.83677548-49
TGCCGTC1113850.020.25382450-51
CCGTCTT1142300.019.75905252-53
Produced by FastQC (version 0.11.5)