FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25152808
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2701711.074118643135192TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG985530.39181708857317243TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA597550.027.93316862-63
GCTTGAA623550.026.87178460-61
GCCGTCT694950.024.06856550-51
ATGCCGT728550.022.99698348-49
GAAAAAA737750.022.84643764-65
GTATGCC742650.022.58851846-47
CGTCTTC755000.022.27168552-53
TCGTATG770050.021.7350944-45
CTGCTTG801350.021.1115658-59
CTTGAAA599250.020.67415462-63
TGAAAAA640300.019.30840564-65
TGCTTGA670500.018.55091960-61
TCTCGTA884800.018.18905442-43
TTCTGCT948450.018.0347256-57
TATGCCG692050.017.827948-49
TCTTCTG978900.017.38145654-55
AATCTCG964050.016.7093440-41
TGCCGTC747600.016.63842450-51
CGTATGC746200.016.53934546-47
CCGTCTT769300.016.14717352-53
Produced by FastQC (version 0.11.5)