FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25261728
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT3820551.5123866427506465TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG861730.34112076576867584TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA757900.028.85293462-63
GCTTGAA768550.028.567660-61
GCCGTCT824650.026.65406850-51
CGTCTTC854150.025.78224652-53
ATGCCGT856950.025.75238648-49
GAAAAAA861000.025.60809964-65
GTATGCC875450.025.17518646-47
TCGTATG895450.024.558444-45
CTTGAAA753150.024.24051962-63
TGAAAAA761550.023.98180664-65
CTGCTTG933150.023.6539758-59
TGCTTGA803250.022.82211160-61
TATGCCG824250.022.17637848-49
TCTCGTA972300.021.73607442-43
TTCTGCT1026600.021.62877856-57
TGCCGTC865900.021.19170250-51
CCGTCTT865300.021.16731852-53
CGTATGC875200.020.85546-47
TCTTCTG1133850.019.63223854-55
GATCTCG1083200.019.51048340-41
Produced by FastQC (version 0.11.5)