FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24460788
Sequences flagged as poor quality0
Sequence length76-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4131521.6890379819325525TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG756070.3090947029179927TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA738500.029.86829462-63
GCTTGAA752900.029.40240960-61
GCCGTCT854450.025.86218550-51
CTTGAAA733100.025.64394262-63
GAAAAAA865900.025.59636164-65
ATGCCGT892150.024.8465648-49
CTGCTTG907450.024.49053658-59
TGAAAAA769450.024.4807664-65
TGCTTGA780750.024.18330460-61
CGTCTTC918200.024.15166952-53
GTATGCC934950.023.66651346-47
TCGTATG954950.023.11974344-45
TATGCCG865400.021.74489648-49
TTCTGCT1067750.020.98748856-57
TGCCGTC906800.020.82100550-51
CGTATGC928100.020.25166946-47
CCGTCTT933850.020.22996752-53
TCTCGTA1062450.020.10105542-43
TCTTCTG1123200.019.88453754-55
CTCGTAT998850.018.73589944-45
Produced by FastQC (version 0.11.5)