FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28795207
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT3115021.0817842010998566TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG1299040.45113063434480605TruSeq Adapter, Index 19 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA761200.027.9540662-63
GCTTGAA782700.027.36450660-61
GCCGTCT808350.026.40452850-51
ATGCCGT839300.025.3565448-49
CGTCTTC843050.025.35412452-53
GTATGCC859200.024.7706246-47
TCGTATG874850.024.28893944-45
GAAAAAA894800.023.94880364-65
CTGCTTG956350.022.50886258-59
TCTCGTA954600.021.41602142-43
TATCTCG986250.020.7132640-41
CTTGAAA763750.020.51011762-63
TTCTGCT1059350.020.37935656-57
TGAAAAA774400.020.30039464-65
TATGCCG798550.019.52014548-49
TGCTTGA810500.019.50736660-61
GCTATCT1094550.018.70265438-39
CCGTCTT850700.018.54408852-53
TGCCGTC861300.018.25290550-51
CGTATGC858650.018.16054546-47
Produced by FastQC (version 0.11.5)