FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30322346
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT4367021.4401985914942068TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG581590.1918024416712348TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA810300.028.09042462-63
GCTTGAA847700.026.97247360-61
GCCGTCT906000.025.2552150-51
CTTGAAA823650.024.87684262-63
TGAAAAA829600.024.79221364-65
CGTCTTC944800.024.2459752-53
GAAAAAA951150.024.11893764-65
ATGCCGT953700.024.02596548-49
GTATGCC970500.023.59995846-47
TGCTTGA886250.023.2917160-61
TCGTATG993900.023.01686544-45
TATGCCG905200.022.69628748-49
CTGCTTG1047000.022.02589258-59
CCGTCTT960150.021.54810152-53
TGCCGTC972350.021.24402450-51
CGTATGC973150.021.1192846-47
TCTCGTA1099500.020.32405742-43
TTCTGCT1167400.019.857656-57
TATCTCG1138350.019.592440-41
CTCGTAT1048550.019.52933344-45
Produced by FastQC (version 0.11.5)