FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31545173
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2986100.9466107540446838TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG1204120.38171291690173964TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA776650.025.88551162-63
GCTTGAA790600.025.58382460-61
GCCGTCT890800.022.63718850-51
GAAAAAA904850.022.3706864-65
ATGCCGT919650.021.91301748-49
GTATGCC955750.021.04634546-47
CGTCTTC974750.020.7432952-53
TCGTATG979500.020.51259244-45
CTGCTTG1001300.020.31710658-59
CTTGAAA775150.018.96791362-63
TGAAAAA793250.018.54615664-65
TTCTGCT1151550.017.79731856-57
TCTCGTA1092050.017.76450542-43
TGCTTGA836850.017.66449460-61
TATCTCG1120450.017.2601540-41
TATGCCG883900.016.51791648-49
TCTTCTG1286300.015.94132454-55
TGCCGTC949050.015.52023850-51
GCTATCT1254950.015.44599538-39
CGTATGC955350.015.2978746-47
Produced by FastQC (version 0.11.5)