FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31877130
Sequences flagged as poor quality0
Sequence length76-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2368530.7430185841699048TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG484560.15200866577386357TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA601950.022.5295662-63
GCTTGAA630250.021.58606760-61
CTTGAAA603400.019.23810462-63
GAAAAAA712500.019.20344464-65
GCCGTCT723000.018.7580750-51
TGAAAAA622250.018.73160664-65
TTACGAC29200.017.8681265
ATGCCGT760450.017.8541948-49
GTATGCC776200.017.44402946-47
GTTACGA30750.017.247914
TGCTTGA694500.016.93788560-61
CGTCTTC813550.016.84563452-53
TCGTATG805250.016.7617444-45
TATGCCG709350.016.25578748-49
CTGCTTG863350.016.00809958-59
CGTTCGA47200.015.3478857
TACGACT35200.015.0674236
TGCCGTC780350.014.96459750-51
CGTATGC778800.014.80283946-47
TGTTACG36850.014.3925133
Produced by FastQC (version 0.11.5)