FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29398765
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3205601.09038593968148TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA660950.024.27538362-63
GCTTGAA680250.023.6845860-61
CTTGAAA659950.022.70507662-63
TGAAAAA680300.022.1210164-65
GAAAAAA789000.020.48868864-65
TGCTTGA738200.020.48494160-61
GCCGTCT824150.019.52947450-51
CGTCTTC885250.018.28913752-53
ATGCCGT885400.018.15655548-49
CTGCTTG897350.018.08230458-59
TATGCCG846150.017.68157648-49
GTATGCC924250.017.37390546-47
TGCCGTC891350.016.91555450-51
CCGTCTT906250.016.6473552-53
TCGTATG964200.016.60301844-45
CGTATGC931350.016.05175246-47
TTCTGCT1052200.015.57285956-57
TTACGAC24300.015.4351875
TCTGCTT989100.015.42638158-59
GTTACGA26200.014.9740434
Produced by FastQC (version 0.11.5)