FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28816229
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2294910.7963949759005594TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG621840.21579506464916004TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA619150.022.24803462-63
GCTTGAA636500.021.62451660-61
GAAAAAA728300.019.06445964-65
CTTGAAA620700.017.7581462-63
GCCGTCT774500.017.71952250-51
TGAAAAA635050.017.41111864-65
CTGCTTG831600.016.73814458-59
ATGCCGT829500.016.54463248-49
TTACGAC25250.016.5333065
TGCTTGA681900.016.24628860-61
CGTCTTC861050.015.99638552-53
GTTACGA26700.015.7966194
ACGTTCG43850.015.7036186
GTATGCC878400.015.60339146-47
CGATCCG46250.015.26392682-83
TCGTATG906650.015.07258444-45
CGTTCGA46550.014.8855657
TTCTGCT964500.014.53227556-57
TGTTACG30800.013.8333643
TATGCCG801850.013.61803248-49
Produced by FastQC (version 0.11.5)