FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28425142
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT6760702.3784225950392788TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG847810.2982606032363884TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA1073550.032.38939362-63
GCTTGAA1121300.031.08832260-61
CTTGAAA1086450.028.83602362-63
GAAAAAA1232750.028.30275564-65
TGAAAAA1115550.028.13994864-65
GCCGTCT1251000.027.90605750-51
ATGCCGT1302400.026.8757748-49
TGCTTGA1174350.026.79817860-61
CTGCTTG1317000.026.58887358-59
GTATGCC1328200.026.34283646-47
CGTCTTC1342450.026.05712152-53
TCGTATG1377200.025.3498244-45
TATGCCG1269550.024.77627648-49
TGCCGTC1311650.024.04009250-51
CGTATGC1343900.023.37089746-47
CCGTCTT1371000.022.94672852-53
TCTTCTG1538250.022.88635354-55
TTCTGCT1540400.022.85445256-57
TCTCGTA1540700.022.15529842-43
TATCTCG1535900.022.12629740-41
Produced by FastQC (version 0.11.5)