FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32075213
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT2493690.777450799781127TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG1317660.4108031956015382TruSeq Adapter, Index 6 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA720650.025.64915762-63
GCTTGAA732600.025.37452360-61
GCCGTCT749050.024.67188350-51
ATGCCGT764150.024.25202848-49
GTATGCC764950.024.20606246-47
TCGTATG780100.023.68433844-45
CGTCTTC798450.023.16195552-53
GAAAAAA867550.021.49739864-65
TCTCGTA867200.020.36594442-43
CTGCTTG990550.018.97605158-59
AATCTCG929350.018.96204240-41
TATGCCG706200.017.7052748-49
CTTGAAA716700.017.60753862-63
TTCTGCT1073100.017.55242556-57
GCGAAAT1027450.017.19027336-37
TGAAAAA738450.017.0831964-65
CGTATGC760100.016.43781946-47
GAAATCT1091550.016.22884238-39
CCGTCTT790050.015.94911552-53
TGCTTGA801750.015.83624560-61
Produced by FastQC (version 0.11.5)