FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004953899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27910091
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2820071.0104123272116885TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG743200.26628361763492636TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)