FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25735077
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1312810.5101247608468396TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG368440.14316646497696509TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA419900.019.95557462-63
GCTTGAA442400.019.03291360-61
GCCGTCT489650.017.1093150-51
GAAAAAA522050.016.2158664-65
ATGCCGT518550.016.14745748-49
CTTGAAA419850.016.04231562-63
GTATGCC535350.015.58368546-47
GTTACGA20650.015.4416614
TGAAAAA437700.015.407701564-65
CGTCTTC556900.015.14804852-53
TCGTATG549400.015.08761144-45
CGTTCGA33650.014.8546657
TTACGAC21600.014.5630215
ACGTTCG34700.014.4049216
TGCTTGA475900.014.26135360-61
CTGCTTG611550.014.01568258-59
TATGCCG477900.013.85084348-49
GTCCGTT33900.013.1079541
CGTATCG28500.013.0024693
GTCCGGT39950.012.9587121
Produced by FastQC (version 0.11.5)