FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33017726
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1845790.55903001920847TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA540250.018.61137862-63
GCTTGAA567400.017.8648260-61
CTTGAAA531950.016.60279862-63
TGAAAAA551100.016.07673364-65
GAAAAAA662400.015.30617964-65
GCCGTCT668550.015.05326350-51
GTCCGAT44000.014.6962671
ATGCCGT698750.014.44890448-49
CGTCTTC700350.014.44696652-53
GTATGCC715850.014.0340146-47
TGCTTGA643200.014.00879960-61
TATGCCG644250.013.58237648-49
TCGTATG747000.013.41460844-45
CTGCTTG810700.012.73751358-59
GTCCGTT46900.012.684541
CCGTCTT710250.012.4448152-53
CGTATCG37750.012.3188413
CGTATGC719100.012.17111446-47
TGCCGTC733250.012.15399750-51
GTCCGGT58600.011.770391
Produced by FastQC (version 0.11.5)