FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31802106
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1753210.5512873895835703TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG881500.27718290103177445TruSeq Adapter, Index 6 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA585850.021.70194662-63
GCTTGAA594350.021.51807460-61
GCCGTCT632050.020.15369450-51
ATGCCGT659150.019.32186748-49
GTATGCC679400.018.68178246-47
CGTCTTC689100.018.49821952-53
TCGTATG685200.018.4612744-45
GAAAAAA718000.017.88153664-65
CTGCTTG794750.016.37968658-59
TCTCGTA786350.015.4485342-43
CTTGAAA562650.015.37438862-63
TTCTGCT903200.014.36054356-57
TGAAAAA604250.014.33375964-65
TATGCCG608200.014.03936948-49
AATCTCG873350.013.93167540-41
CGTTCGA31000.013.8859037
TGCTTGA641850.013.6749860-61
ACGTTCG32100.013.4098956
GTCCGTT40350.012.9266851
CCCGTAT40700.012.9214351
Produced by FastQC (version 0.11.5)