FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33212740
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1682900.5067031506584522TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG479650.14441747353575765TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA591500.017.39484262-63
GCTTGAA633500.016.45901760-61
GAAAAAA719200.014.60862964-65
GTCCGAT40550.014.0383851
CTTGAAA601650.013.6252662-63
TGAAAAA611050.013.4966564-65
GCCGTCT773350.013.28834650-51
CGTCTTC788550.013.09540352-53
GTCCGGT54400.012.6046931
ATGCCGT831850.012.31500148-49
CTGCTTG856250.012.30339258-59
TGCTTGA692150.012.09255260-61
CCCGTAT41500.011.8465211
CGTATCG28300.011.7200833
GTATGCC880450.011.63474346-47
CCGTATC44150.011.1225022
TCGTATG930100.010.98156744-45
TTCTGCT1008850.010.61536556-57
GTCGGTT54100.010.5223021
GTCCGAA48400.010.5140661
Produced by FastQC (version 0.11.5)