FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33798192
Sequences flagged as poor quality0
Sequence length76-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT4220031.2485963746226425TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG443260.13114902714322707TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA826950.026.47355562-63
GCTTGAA852550.025.7566760-61
CTTGAAA824750.024.35830562-63
TGAAAAA843050.023.8858964-65
GCCGTCT937350.023.35625550-51
GAAAAAA973200.022.66363164-65
CGTCTTC969200.022.64914752-53
TGCTTGA899500.022.46323260-61
ATGCCGT989850.022.10663448-49
GTATGCC1026100.021.30383146-47
TATGCCG946850.021.1359248-49
TCGTATG1052950.020.70191844-45
CCGTCTT976850.020.62396452-53
CTGCTTG1087200.020.3032558-59
TGCCGTC1012700.019.90207550-51
CGTATGC1025400.019.51187746-47
TTCTGCT1192800.018.58898456-57
TCTCGTA1161950.018.18810842-43
CTCGTAT1111850.017.9430244-45
TCTGCTT1149450.017.63716158-59
Produced by FastQC (version 0.11.5)