FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004953928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73754438
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15326132.0779942760868164No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACGGATCTCGTA1547790.20985720208457154Illumina Paired End PCR Primer 2 (97% over 38bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1043290.14145453864077984No Hit
TTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT766660.10394764312352295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)