FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004954707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004954707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51995255
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4478630.8613535985158646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT72150.016.1922455
TCTTCCG88850.013.6015723
TCCGATC86300.013.4685326
CCGATCT89600.012.6383037
CTTCCGA93150.012.637584
CGATCTA17650.09.0930288
CGTTGGA68650.08.2326891
GCTCTTC176650.07.37474541
CGATCTT45500.06.4282498
ACGGGAT51500.06.20369779
AACGGGA76350.06.0114868
AAACGGG97250.05.9068917
CGATCTC95400.05.83304078
GAAACGG106950.05.7495316
GGAAACG113250.05.6147915
CGATCTG37200.05.60455758
GTTGGAA136450.05.4603282
CCCGAAT36000.05.330261
CCCTAAA101400.05.26323751
CCCAAGT152050.05.1466541
Produced by FastQC (version 0.11.5)