FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004954708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004954708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523812
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGTCATGGG943173.737085012671308TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGTCATGTG110670.4385033433552103TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGTCATGGC99960.39606753593373833TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGTCAGGGG53470.21186205628628438TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGTCATCGG51040.2022337638461185TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30930.12255270994828457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGTCATGTC29860.11831309146640083TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)